Avtor/Urednik		Jeršek, B; Gilot, P; Gubina, M; Klun, N; Mehle, J; Tcherneva, E; Rijpens, N; Herman, L
Naslov		Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR
Tip		članek
Vir		J Clin Microbiol
Vol. in št.		Letnik 37, št. 1
Leto izdaje		1999
Obseg		str. 103-9
Jezik		eng
Abstrakt		Listeria monocytogenes strains posses short repetitive palindromic (REP) elements and enterobacterial repetitive intergenic consensus (ERIC) sequences. We used repetitive element sequence-based PCR (rep-PCR) to evaulate the potential of REF and ERIC elements for typing. L. monocytogenes strains isolated from humans, animals, and food. On the basis of rep-PCR fingerprints, L. monocytogenes strains were divided into four clusters matching origin of isolation. rep-PCR fingerprints of human and animal isolated were different from those of food isolates. Computer evaulation of rep-PCR fingerprints allowed discrimination among the tested serotypes 1/2a, 1/2b, 1/2c, 3b, and 4b within each major cluster. The index of discrimination calculated for 52 epidemiologically unrelated isolates of L. monocytogenes was 0.98 for REP and ERIC-PCR. Our results suggest that rep-PCR can provide an alternative method for L. monocytogenes typing.
Deskriptorji		LISTERIA MONOCYTOGENES POLYMERASE CHAIN REACTION REPETITIVE SEQUENCES, NUCLEIC ACID SEROTYPING GENOME, BACTERIAL FOOD MICROBIOLOGY DNA FINGERPRINTING