Author/Editor | Jeršek, B; Gilot, P; Gubina, M; Klun, N; Mehle, J; Tcherneva, E; Rijpens, N; Herman, L | |
Title | Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR | |
Type | članek | |
Source | J Clin Microbiol | |
Vol. and No. | Letnik 37, št. 1 | |
Publication year | 1999 | |
Volume | str. 103-9 | |
Language | eng | |
Abstract | Listeria monocytogenes strains posses short repetitive palindromic (REP) elements and enterobacterial repetitive intergenic consensus (ERIC) sequences. We used repetitive element sequence-based PCR (rep-PCR) to evaulate the potential of REF and ERIC elements for typing. L. monocytogenes strains isolated from humans, animals, and food. On the basis of rep-PCR fingerprints, L. monocytogenes strains were divided into four clusters matching origin of isolation. rep-PCR fingerprints of human and animal isolated were different from those of food isolates. Computer evaulation of rep-PCR fingerprints allowed discrimination among the tested serotypes 1/2a, 1/2b, 1/2c, 3b, and 4b within each major cluster. The index of discrimination calculated for 52 epidemiologically unrelated isolates of L. monocytogenes was 0.98 for REP and ERIC-PCR. Our results suggest that rep-PCR can provide an alternative method for L. monocytogenes typing. | |
Descriptors | LISTERIA MONOCYTOGENES POLYMERASE CHAIN REACTION REPETITIVE SEQUENCES, NUCLEIC ACID SEROTYPING GENOME, BACTERIAL FOOD MICROBIOLOGY DNA FINGERPRINTING |