Author/Editor		Poljak, Mario; Seme, Katja
Title		Virološka diagnostika okužbe s HIV
Type		članek
Source		In: Bregant L, editor. Zbornik referatov Nebakterijske okužbe v perinatologiji; 1998 maj 8-9; Ljubljana. Ljubljana: Društvo za pomoč prezgodaj rojenim otrokom,
Publication year		1998
Volume		str. 72-6
Language		slo
Abstract		Current routine diagnosis of human immunodeficiency virus (HIV) infection is based on the detection of specific antiviral antibodies in body fluids, mainly serum samples. Enzyme immunoassays and agglutionation tests are most widely used basic screening seological methods. Since the specificity of screening tests is limited, all anti-HIV reactive screening test results have to be confirmed, using confirmatory tests such as Western-blot or immunoblot tests. Diagnostic problems which can not be resolved with indirect serological methods were used to be cleared by using two classical methods for direct detection of viral infection: p24 viral antigen assay or HIV culture. Polymerase chain reaction (PCR) and other amplification methods are currently the methods of choice for direct detection of HIV infection. Qualitative PCR in which parts of HIV proviral DNA integrated inhuman genome are detected is mainly used for resolving of HIV infection status in individuals with indeterminate Western-blot results. Quantitative amplification methods which provide a quantitative evaluation of the number of copies of the RNA genome present in blood is mainly used as an important prognostic marker ofdisease progression and for rapid evaluation of patient's response to antiretroviral treatment.
Descriptors		HIV INFECTIONS HIV SERODIAGNOSIS HIV ANTIBODIES HIV SEROPOSITIVITY POLYMERASE CHAIN REACTION