Author/Editor | Ihan, Alojz | |
Title | A correlation of NK cytolitic test and BLT esterase test in determining activity of NK cells, stimulated by tumor target cells | |
Translated title | Korelacija med NK citolitičnim in BLT esteraznim testom pri določevanju aktivnosti NK celic stimuliranih s tumorskimi celicami | |
Type | članek | |
Source | Radiol Oncol | |
Vol. and No. | Letnik 33, št. 1 | |
Publication year | 1999 | |
Volume | str. 27-33 | |
Language | eng | |
Abstract | We examined the granule exocytosis in natural killer (NK) cells by measuring N-benzyloxycarboxy-L-lysine esterase activity. As a source of NK cells we used buffy-coat isolated NK cells or peripheral blood lymphocytes (PBL). The exocytosis was triggered by incubating cells with ionomycin/PMA or by NK cell susceptible tumour target cells K562. When we stimulated purified NK cells with tumour target cells, a close correlation (Corr=0.84) of cytolitic NK test results and BLT test results was obtained. We may conclude that BLT test can provide a valuable tool to discriminate further NK cell deficiency in patients with low cytolitic NK test results. | |
Summary | Z meritvami encimske aktivnosti N-benzyloxycarboxy-L-lizinske esteraze smo preiskovali eksoitozo celic NK. Celice NK smo osamili iz levkocitnega koncentrata (Buffy coat) oz. venske krvi. Eksocitozo smo sprožili s kombinacijo PMA/ionomcin ali s tumorskimi celicami K542. Ob stimulaciji celic NK s tumorskimi celicami (K542) smo opazovali značilno soodvisnost (Cor=0.H4) med rezultati citolitične aktivnosti celic NK in med izmerjenimi encimskimi aktivnosti N-benzv- loxycarboxy-L-lizinske esteraze (BLT test). Ugotavljamo, da BLT test omogoča nadrobnej5i študij dogodkov, ki se odvijajo med citotoksično aktivnostjo celic NK. | |
Descriptors | KILLER CELLS, NATURAL EXOCYTOSIS LYMPHOCYTES ESTERASES SERINE TUMOR CELLS, CULTURED CYTOTOXICITY TESTS, IMMUNOLOGIC FLOW CYTOMETRY CELL DEGRANULATION |