Avtor/Urednik		Falnoga, I; Tušek-Žnidarič, M; Milačič, R
Naslov		Mercury and methallothionein-like proteins in the particulate cell fraction of human cerebellar nucleus dentatus
Tip		članek
Vir		Acta Chim Slov
Vol. in št.		Letnik 45, št. 3
Leto izdaje		1998
Obseg		str. 229-37
Jezik		eng
Abstrakt		The particulate fraction (pellet) of human cerebellar nucleus dentatus from a retired mercury mine worker with a high mercury and selenium content (2.35 microg/g, 1.02microg/g) was subjected to mild extraction of Hg binding proteins with a buffer containing 10% mercaptoethanol (ME). The amount of mercury solubilised in this procedure was very low, about 3% of the pellet Hg content. Nevertheless, in the ME extract the presence of metallothionein - like proteins (MT-LP) were found by Sephadex G-75 gel chromtography and subsequent metal analysis. There were detected as Cu, Zn, Pb, Hg MT-LP, where the binding of Cu was much the highest and Hg was present only in traces. These data indicate that in accordance with literature discussions MT in nucleus dentatus could be involved in Cu metabolism and in short term heavy metal detoxification (Pb, Hg).
Deskriptorji		MERCURY METALLOTHIONEIN CEREBELLAR NUCLEI