Author/Editor		Smole-Možina, S; Rozman, D; Komel, R; Raspor, P
Title		Identification and genetic inter-relationship analysis of Saccharomyces yeasts by arbitrary primed PCR fingerprinting
Type		članek
Source		Acta Chim Slov
Vol. and No.		Letnik 42, št. 4
Publication year		1995
Volume		str. 459-64
Language		eng
Abstract		In the present investigation, we used arbitrary primed polymerase chain reaction (AP - PCR) to generate genomic fingerprints of Saccharomyces type strains and other isoaltes by several decamer oligonucleotide primers (50 - 90% G+C, promega, Operon) and microsatellite DNA primers ((GTG)5X (GACA)4X, MWG - Biotech. Genotypic relatedness of yeasts, evaluated on the basis of polymorphic RAPD markers, was expressed as calculated genetic similarity coefficients and cluster analysis based on UPGMA method (NTSYS pc program). The estimated similarity values were between 43% - 88% for Saccharomyces sensu stricto strains, but much lower fro Saccharomyces sensu lato yeasts. The results revealed high differentiation capacity of AP-PCR analysis in identification studies of very closely related strains of yeasts from the same species and good correlation of calculated relatedness with the results of mroe sophisticated molecular methods.
Descriptors		SACCHAROMYCES DNA PRIMERS MICROSATELLITE REPEATS POLYMERASE CHAIN REACTION YEASTS ELECTROPHORESIS, AGAR GEL POLYMORPHISM (GENETICS) CLUSTER ANALYSIS