Avtor/Urednik		Sočan, M; Marinič-Fišer, N; Keše, D
Naslov		Comparison of serologic tests with urinary antigen detection for diagnosis of legionnaires' disease in patients with community-acquired pneumonia
Tip		članek
Vir		Clin Microbiol Infect
Vol. in št.		Letnik 5, št. 4
Leto izdaje		1999
Obseg		str. 201-4
Jezik		eng
Abstrakt		Objective: To compare serologic methods and detection of urinary antigen in the diagnosis of community-acquired pneumonia. Methods: Paried sera from 84 patients with community-acquired pneumonia were tested for Legionella pneumophila serogroup (LP SG) 1-7 and Legionella micdadei by use of the indirect immunofluorescence antibody test (IIF), enzyme-linked immunosorbent assay (ELISA) for LP SG 1-7 and complement-fixation (CF) test for LP SG 1. All patients were evaluated by ELISA urinary antigen detection for LP SG 1. Results: Seven patients met the CDC criteria for acute Legionella infection, while in the rest of them we failed to detect urinary Legionella antigen. Thirty-three patients had non-diagnostic IIF antibody titers. Serum ELISA (IgG and/or IgM) was positive in 40 patients. Nine patients showed at least one CF titer of >= 1:32. The sensitivities of ELISA IgM for the first and the second serum samples compared with IIF were 42.8% and 46.6%, respectively, while the specificities were higher, i.e. 87% and 88,4% respectively. The sensitivities of ELISA IgG for the first and the second samples were 42,8% and 53,3%, and the specificities were 77,9% and 76,8%, respectively. Conclusions: Although ELISA is simple to perform and easy to automate, we think that its advantages over indirect immunofluorescence and urinary antigen detection remain questionable.
Deskriptorji		PNEUMONIA LEGIONNAIRES' DISEASE LEGIONELLA PNEUMOPHILA COMORBIDITY FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT ENZYME-LINKED IMMUNOSORBENT ASSAY COMPLEMENT FIXATION TESTS ANTIGENS, BACTERIAL IGM IGG