Avtor/Urednik		Werle, B; Loetterle, H; Schanzenbaecher, U; Lah, TT; Kalman, E; Kayser, K; Buelzebruck, H; Schirren, J; Krašovec, M; Kos, J
Naslov		Immunochemical analysis of cathepsin B in lung tumours: an independent prognostic factor for squamous cell carcinoma patients
Tip		članek
Vir		Br J Cancer
Vol. in št.		Letnik 81, št. 3
Leto izdaje		1999
Obseg		str. 510-9
Jezik		eng
Abstrakt		In order to evaluate the possible role of the proteolytic enzyme cathepsin B (cath B) in human non-small cell lung cancer (NSCLC) we examined cath B concentrations (cath Bc) and activities (cath Ba) in homogenates of 127 pairs of lung tumour tissues and corresponding non-tumourous lung parenchyma. Total cath B activity (cath B AT) and enzymatic activity of the fraction of cath B, which is stable and active at pH 7.5 (cath B A7.5) were determined by a fluorogenic assay using synthetic substrate Z-Arg-Arg-AMC. The immunostaining pattern of cath B was determined in 239 lung tumour tissue sections, showing the presence of the enzyme in tumour cells (cath B T-I) and in tumour-associated histiocytes (cath B H-I). The median levels of cath B AT, cath B A7.5 and cath B C were 5.6-, 3.2- and 9.1-fold higher (P< 0.001 ), respectively, in tumour tissue than in non-tumourous lung parenchyma. Out of 131 tissue sections from patients with squamous cell carcinoma (SCC), 59.5% immunostained positively for cath B, while among the 108 adenocarcinoma (AC) patients 48.2% of tumours showed a positive reaction. There was a strong relationship between the levels of cath B AT, cath B A7.5, cath B C and cath B T-I in the primary tumours and the presence of lymph node metastases. Significant correlation with overall survival was observed for cath B T-I and cath B A7.5 (P < 0.01 and P < 0.05, respectively) in patients suffering from SCC. In these patients positive cath B in tumour cells (cath B T-I) and negative cath B in histiocytes (cath BT-I) indicated significantly shorter survival rate compared with patients with negative cath B T-I and positive cath B H-I (P< 0.0001). In contrast, in AC patients, both, positive cath B T-I and positive cath B H-I, indicated poor survival probability (P < 0.014). From these results we conclude that the proteolytic enzyme cath B is an independent prognostic factor for overall survival of patients suffering from SCC of the lung.
Deskriptorji		LUNG NEOPLASMS CARCINOMA, NON-SMALL-CELL LUNG CARCINOMA, SQUAMOUS CELL CATHEPSIN B NEOPLASM PROTEINS ADENOCARCINOMA NEOPLASM STAGING PROGNOSIS TUMOR MARKERS, BIOLOGICAL SURVIVAL ANALYSIS