Avtor/Urednik		Polanec, J; Pavelič, ZP; Myers, WL
Naslov		Effect of Wnt-1 antisense RNA on the outgrowth of a mammary adenocarcinoma cell line expressing that oncogene
Tip		članek
Vir		J Clin Pathol
Vol. in št.		Letnik 49
Leto izdaje		1996
Obseg		str. M166-69
Jezik		eng
Abstrakt		Aims - To investigate the effect of Wnt-1 antisense RNA on the outgrowth of a mammary tumour cell line expressing that oncogene. Methods - A plasmid (pMT 70), containing Wnt-1 cDNA, was cut with appropriate enzymes and inserted into a eukaryotic expression vector (pMAMneo). A mammary tumour cell line (CAC-L153) was transfected with the expression vector and cells with the vector in sense and antisense orientation were selected. Results - Tumour cells with the expression vector ia the antisense orientation had a notable reduction in expression of Wnt-1 protein and a considerable reduction in tumour outgrowth compared with controls. Conclusions - The results indicate that the Wnt-1 proto-oncogene may be a possible target for antisense therapy.
Deskriptorji		TUMOR CELLS, CULTURED MAMMARY NEOPLASMS MAMMARY TUMOR VIRUSES, MOUSE MUTAGENESIS, INSERTIONAL RNA, ANTISENSE PROTO-ONCOGENES