Author/Editor		Cankar, Ksenija
Title		Vloga adrenergičnih receptorjev alfa pri mikrocirkulacijskem odgovoru kože na hlad in zažem arterij
Type		monografija
Place		Ljubljana
Publisher		Univerza v Ljubljani, Medicinska fakulteta
Publication year		2000
Volume		str. 75
Language		slo
Abstract		Clinically most often used methods for evaluation of the microcirculatory function are response to cold and to arterial occlusion. Both responses are complex in nature. They are composed of several either local or systemic components. Their roles have not been clarified yet. According to the previous studies, there is an unsolved question on the contribution of the sympathetic nervous system acting thru the alpha1 and alpha2 adrenergic receptors. Our aim was to clarify the role of these receptors in the origin of both microcirculatory responses with the use of the laser-Doppler method for skin flow measurements. In the first part of the study we compared responses to the direct and indirect (the cooling of contralateral hand) local cooling before and after intradermal injection of the alpha-adrenoceptor antagonists and agonists with the response in the denervated replanted fingers. In the second part we compared cutaneous postocclusive reactive hyperaemia (PRH before and after intradermal injection of the alpha-adrenoceptor antagonists and agonists. METHODS: Laser-Doppler (LD) flow measurements were performed on the fingertips of healthy volunteers and of patients with replanted digits. LD flow was monitored 2 minutes before and 6 minutes during local cooling of one hand at 15 degrees Celsius and before and after an 8-minute occlusion of digital arteries. In healthy fingers, we studied the effect of intradermal microinjection (1 micro l) of alpha antagonists (prazosine, yohimbine) and alfa agonists (phenilephrine, clonidine) on local cooling and PRH responses. We evaluated the degree of the LD flow decrease in response to the direct and indirect local cooling with respect to the values obtained before cooling (ANOVA, Dunnet t-test). We analysed the magnitude of peak flow of PRH and its duration. (Abstract truncated at 2000 characters).
Descriptors		SKIN MICROCIRCULATION COLD HYPEREMIA RECEPTORS, ADRENERGIC, ALPHA SYMPATHETIC NERVOUS SYSTEM LASER-DOPPLER FLOWMETRY ADRENERGIC ALPHA-AGONISTS ADRENERGIC ALPHA-ANTAGONISTS SKIN TEMPERATURE ELECTROCARDIOGRAPHY VASODILATION