Author/Editor		Franko, Mladen; Šikovec, Mateja; Kožar-Logar, Jasmina
Title		Optotermična detekcija v gradientni HPLC
Type		članek
Source		In: Glavič P, Brodnjak-Vončina D, editors. Zbornik referatov s posvetovanja Slovenski kemijski dnevi 2000: 1. in 2. del; 2000 sep 28-29; Maribor. Maribor: FKKT,
Publication year		2000
Volume		str. 351-5
Language		slo
Abstract		Thermal lens spectrometry belongs to the group of photo-thermal techniques, which due to its inherent high sensitivity enables detection of various pigments such as chlorophyll or various carotenoids at concentrations below 1 microgL-1. This work represents the application of the TLS detection in combination with a gradient HPLC. While gradient elution provides the required selectivity of analytical procedure, the high sensitivity of TLS detection facilitates the determination of different compounds, and adds to the accuracy of the discrimination between representative species. This fact enables the use of HPLC-TLS method for determination of carotenoids and chlorophylls in complex samples like various species of marine phytoplankton.
Descriptors		CHROMATOGRAPHY, HIGH PRESSURE LIQUID SPECTRUM ANALYSIS TEMPERATURE CAROTENOIDS CHLOROPHYLL PHYTOPLANKTON SEAWATER