Avtor/Urednik		Frangež, Robert; Križaj, Igor; Gubenšek, Franc; Šuput, Dušan
Naslov		Effects of ammodytin L on miniature and endplate potentials in neuromuscular junction of frog m. cutaneus pectoris
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 440, št. 5 Suppl
Leto izdaje		2000
Obseg		str. R101-2
Jezik		eng
Abstrakt		Neurotoxic effects of ammodytin L (AtnL) a potent phospholipase A2 homologue, was studied in frog neuromuscular preparation m. cutaneus pectoris by measuring the influence of the toxin on the amplitude and the frequency of miniature and endplate potentials (MEPPs, EPPs). Atnl, in 100 nM concentration, significantly increases spontaneous quantal acetylcholine release from the motor nerve endings, observed as the increase in MEPPs frequency. At 100 nM or higher concentration the toxin decreases EPPs amplitude and the membrane potential (MP) simultaneously. No significant effect of AtnL on EPPs was observed at any concetration bellow 100 nM. Our results indicate that in frog AtnL shows the typical myotoxic effects, but it also exerts presynaptic effects.
Deskriptorji		ACTION POTENTIALS PHOSPHOLIPASES A NEUROMUSCULAR JUNCTION MUSCLE, SKELETAL MOTOR ENDPLATE VIPER VENOMS RANA ESCULENTA