Avtor/Urednik		Comino, Aleksandra; Manček, Mateja; Komel, Radovan
Naslov		Ecm11 is located in the cell nucleus throughout mitosis
Tip		članek
Vir		Pflugers Arch
Vol. in št.		Letnik 442, št. 6 Suppl 1
Leto izdaje		2001
Obseg		str. R179-81
Jezik		eng
Abstrakt		It was found previously that Ecm11 interacts with Cdc6 in a two-hybrid screen. To verify that these two gene products can actually encounter in the living cell, ECM11 was,.expressed as a fusion with GFP. The fusion protein was located in the cell nucleus throughout all stages of mitosis. The protein was detected in discrete clusters in the nucleus. Clusters may be due to Ecm11 protein polymerisation and association with discrete structures in the nucleus. Two Ecm11 molecules interact in the two-hybrid assay, suggesting the presence of functional dimmers. Ecm11 does not promote transcription of beta-gadactosdase reporter :gene, when fined with LexA DNA binding domain and fetch to the promoter of the reporter gene. According to our observation; we suppose that Ecm11 is a nuclear protein , involved in the upholding chromatin structure.
Deskriptorji		GENES, CDC CELL CYCLE PROTEINS TRANSCRIPTION, GENETIC CHROMATIN MITOSIS SACCHAROMYCES PLASMIDS HYBRIDIZATION BETA-GALACTOSIDASE