Author/Editor		Petrovič, Nataša; Šoster, Petra; Korošec-Koruza, Zora; Ravnikar, Maja; Meng, Bao Zhong; Gonsalves, Dennis
Title		Prvi rezultati uporabe novih laboratorijskih metod za ugotavljanje zastopanosti virusa, ki povzroča razbrazdanje lesa na vinski trti v Sloveniji
Translated title		First results on the use of laboratory methods for detection of rupestris stem pitting associated virus 1 in grapevines in Slovenia
Type		članek
Source		In: Dobrovoljc D, Urek G, editors. Zbornik predavanj in referatov 5. slovensko posvetovanje o varstvu rastlin; 2001 mar 6-8; Čatež ob Savi. Ljubljana: Društvo za varstvo rastlin Slovenije,
Publication year		2001
Volume		str. 468-72
Language		slo
Abstract		We are introducing laboratory methods for a detection of the causal agent of rupestris stem pitting disease, a rupestris stem pitting associated virus 1 (RSPaV-1): serological methods (ELISA and Western blot) and RT-PCR. The techniques have generated some interesting preliminary results on the presence of the RSPaV-1 in indicaor vines of V. rupestris cv. St. George, and in V. vinifera cv. Refošk. Analyses showed the infection of six (out of six analysed) St. George plants, and the infection of four (out of five analysed) Refošk vines. Interestingly, all infected St. George vines and two of the positively analysed Refošk vines show no symptoms of rugose wood.
Descriptors		FRUIT PLANT VIRUSES ENZYME-LINKED IMMUNOSORBENT ASSAY POLYMERASE CHAIN REACTION BLOTTING, WESTERN