Author/Editor		Petrovič, Nataša; Jeraj, Nataša; Ravnikar, Maja
Title		Uporaba tkivnih kultur za izboljšanje detekcije fitoplazem v vinski trti
Translated title		The use of tissue culture for improved detection of phytoplasma in grapevines
Type		članek
Source		In: Dobrovoljc D, Urek G, editors. Zbornik predavanj in referatov 5. slovensko posvetovanje o varstvu rastlin; 2001 mar 6-8; Čatež ob Savi. Ljubljana: Društvo za varstvo rastlin Slovenije,
Publication year		2001
Volume		str. 489-91
Language		slo
Abstract		Heterogenous distribution, low concentration and seasonal variations of grapevine yellows phytoplasma in grapevines remain as main problems which prevent the introduction and standardization of molecular biology-based quick laboratory detection protocols for their routine use in certification and quarantine. The results showed increased phytoplasma concentration and homogenous distribution in infected Vitis vinifera and Catharanthus roseus grown in tissue culture in comparison to plants grown in soil. Present results indicate the potential use of tissue culture as an alternative tool in improving laboratory methods of phytoplasma detection in grapevines, especially in detecting extremely low concentrations of phytoplasma in specific samples of grapevine, and in discovering latent infections.
Descriptors		FRUIT TISSUE CULTURE FUNGI