Avtor/Urednik		Stanič-Racman, Darja; McGeachy, Kara; Reavy, Brian; Štrukelj, Borut; Žel, Jana; Barker, Hugh
Naslov		Strong resistance to potato tuber necrotic ringspot disease in potato induced by transformation with coat protein gene sequences from an NTN isolate of Potato virus Y
Tip		članek
Vir		Ann Appl Biol
Vol. in št.		Letnik 139
Leto izdaje		2001
Obseg		str. 269-75
Jezik		eng
Abstrakt		The potato cv. Igor is susceptible to infection with Potato virus Y (PVY) and in Slovenia it has been so severely affected with NTN isolates of PVY causing potato tuber necrotic ringspot disease (PTNRD) that its cultivation has ceased. Plants of cv. Igor were transformed with two transgenes that contained coat protein gene sequence of PVYntn. Both transgenes used PVY sequence in a sense (+) orientation, one in native translational context (N-CP), and one with a frame-shift mutation (FS-CP). Although most transgenic lines were susceptible to infection with PVYNTN and PVY0, several lines showed resistance that could be classified into two types. Following manual or graft inoculation, plants of partially resistant lines developed some symptoms in foliage and tubers, and virus titre in the foliage, estimated by ELISA, was low or undetectable. In highly resistant (R) lines, symptoms did not develop in foliage and on tubers, and virus could not be detected in foliage by ELISA or infectivity assay. Four lines from 34 tested (two N-CP and two FS-CP) were R to PVYNTN and PVY0 and one additional line was R to PVY). When cv. Spey was transformed with the same constructs, they did not confer strong resistance to PVY0.
Deskriptorji		POTATOES POTYVIRUS PLANTS, TRANSGENIC ENZYME-LINKED IMMUNOSORBENT ASSAY TISSUE CULTURE DNA RNA POLYMERASE CHAIN REACTION