Avtor/Urednik		Trček, Janja; Teuber, Michael
Naslov		Genetic and restriction analysis of the 16S-23S rDNA internal transcribed spacer regions of the acetic acid bacteria
Tip		članek
Vir		FEMS Microbiol Lett
Vol. in št.		Letnik 208, št. 1
Leto izdaje		2002
Obseg		str. 69-75
Jezik		eng
Abstrakt		The 16S-23S rDNA internal transcribed spacer regions of the acetic acid bacteria were sequenced and evaluated for molecular identification of these bacteria. All the sequenced spacers contained genes for tRNAIle and tRNAAla, and the antitermination element. The sequences revealed 56.8-78.3% similarity. By PCR amplification of the spacers from 57 strains of acetic acid bacteria, single products of similar sizes were produced. Digestion of the spacers by HaeIII and HpaII restriction enzymes resulted in 12 distinct groups of restriction types. All the restriction profiles obtained after analysis of microbial populations from vinegar matched one of the 12 groups.
Deskriptorji		ACETOBACTERACEAE POLYMORPHISM, RESTRICTION FRAGMENT LENGTH DNA, RIBOSOMAL BASE SEQUENCE POLYMERASE CHAIN REACTION