Author/Editor		Drevenšek, Petra; Golobič, Amalija; Turel, Iztok; Poklar, Nataša; Sepčić, Kristina
Title		Crystal structure, characterisation and biological activity of copper(II)-ciprofloxacin ionic compound
Type		članek
Source		Acta Chim Slov
Vol. and No.		Letnik 49
Publication year		2002
Volume		str. 857-70
Language		eng
Abstract		The title compound, ciprofloxacinium(1+) ciprofloxacinium(2+) tetrachlorocuprate(In chloride hydrate, (cfH2)(cfH3)[CuCl4]CfH2O, (1), was isolated and its structure was determined by X-ray crystallography. This is a typical ionic compound with no direct bonds between the quinolone and metal. Both ciprofloxacin {cfH = 1-cyclopropyl-6-fluoro-l,4dihydro-4-oxo-7-(1-piperazinyl)-3-quin oline carboxylic acid) molecules are nonequivalent and are protonated thus being unable to coordinate to the copper. The new complex has been characterised by intrinsic fluorescence emission and UV spectroscopy. The title compound as well as two previously isolated copper complexes, [Cu(cfH)(H2O)3]SO42H2O, (2) and [Cu(cfH)2]Cl26H2O, (3), were tested against the growth of various Gram positive and Gram negative microorganisms. Antimicrobial activities were evaluated using the agar diffusion test. Since ciprofloxacin alone has the ability to bind to DNA, the binding of a new cornpound has also been tested by UV spectroscopy. Our results reveal that (cfH2)(cfH3)[CuCl4]CIH2O slightly thermally destabilise the linear double stranded DNA at pH 7.0.
Descriptors		COPPER CIPROFLOXACIN CRYSTALLOGRAPHY, X-RAY MOLECULAR CONFORMATION BACTERIA QUINOLONES HYDROGEN BONDING HYDROGEN-ION CONCENTRATION DNA