Avtor/Urednik		Kožar-Logar, Jasmina; Malej, Alenka; Franko, Mladen
Naslov		Hyphenated high perfomance liquid chromatography-thermal lens spectrometry technique as a tool for investigations of xanthophyll cycle pigments in different axonomic groups of marine phytoplankton
Tip		članek
Vir		Rev Sci Instrum
Vol. in št.		Letnik 74, št. 1
Leto izdaje		2003
Obseg		str. 776-8
Jezik		eng
Abstrakt		Hyphenated high performance liquid chromatography-thermal lens spectrometry (HPLC-TLS) was applied to the determination of carotenoids in four phytoplankton species. Satisfactory separation of diadinoxanthin, diatoxanthin, and other carotenoids was achieved by isocratic HPLC elution providing 40-80 times lower LOD, compared to UV Vis detector. Besides the interconversion of diadinoxanthin to diatoxanthin, changes of other minor carotenoids under different light conditions were possible to follow. The isocratic HPLC-TLS provides sufficient sensitivity and selectivity for studies of photochemical processes in marine phytoplankton involving pigments, which occur in traces.
Deskriptorji		CHROMATOGRAPHY, HIGH PRESSURE LIQUID ALCOHOL, METHYL ACETONE AMMONIUM COMPOUNDS TEMPERATURE REFRACTOMETRY