Avtor/Urednik | Tomažič, M; Janež, A; Sketelj, A; Kocijančič, A; Eckel, J; Sharma, PM | |
Naslov | Comparison of altreations in insulin signalling pathway in adipocytes from type II diabetic women and women with gestational diabetes mellitus | |
Tip | članek | |
Vir | Diabetologia | |
Vol. in št. | Letnik 45 | |
Leto izdaje | 2002 | |
Obseg | str. 502-8 | |
Jezik | eng | |
Abstrakt | Aims/hypotlhesis. The cellular mechanisms for the insulin resistance in pregnancy and gestational diabetes mellitus are not known. The membrane protein plasma cell glycoprotein PC-l has been identified as an inhibitor of insulin receptor tyrosine kinase activity and could have a role in insulin resistance. This study aimed to examine the effects of insulin on glucose transport and changes in insulin receptor tyrosine phosphorylation, IRS-1 and PC-1. Method,s. Adipocytes were obtained either during electivc cesarean section from three groups of subjects (Type II diabetic pregnant women (n=6) women with gestational diabetes mellitus (n=10) and pregnant women with normal glucose tolerance (n=C) as pregnant control subjects) or during elective gynaecological surgery from non-pregnant (n=6) control subjects. Results. Insulin stimulated glucose transport was reduced by 50% in women with gestational diabetes mellitus and 70% in pregnant women with Type II diabetes, compared to the non-pregnant control subjects. After maximal insulin stimulation of adipocytes, 1RTK phosphorylation was reduced by 29.5% in women with gestational diabetes mellitus and 44.5% in women with Type II diabetes, compared to the nonpregnant control subjects. We also found that IRS-1 phosphorylation was reduced by 32% and 48%, respectively. On the other hand, PC-1 content in adipocytes in women with gestational diabetes rnellitus increased by 320% and 668% in Type II diabetic women, compared to the non-pregnant control subjects. Conclusions/interpretation. Our results indicate that women with gestational diabetes mellitus and Type II diabetes have increased PC-1 content and suggest that this could contribute to lower phosphorylation levels of IRTK and IRS-l. (Abstract truncated at 2000 characters). | |
Deskriptorji | DIABETES MELLITUS, NON-INSULIN-DEPENDENT DIABETES, GESTATIONAL ADIPOCYTES INSULIN INSULIN RESISTANCE PREGNANCY INSULIN RECEPTOR PROTEIN-TYROSINE KINASE GLUCOSE PHOSPHORYLATION P-GLYCOPROTEIN |