Author/Editor		Štempelj, M; Čarman-Kržan, M; Ferjan, I
Title		Regulatory role of extracellular Na+ and Ca+ ions in nerve growth factor induced histamine secretion from rat mast cells
Type		članek
Source		Inflamm Res
Vol. and No.		Letnik 52
Publication year		2003
Volume		str. 74-8
Language		eng
Abstract		Objective and design: This study was aimed to investigatc effccts of extracellular Na+ and Ca2+ ions on ncrvc growth factor (NGF) induced histamine release from mast cells. Material: Isolated peritoneal mast cells were obtained from male Wistar rats. Methods: Cells were suspended in solution with different concentration of Na+ and Ca2+ ions and stimulated with NGF Histamine release was assayed spectrofluorometrically. Results: NGF (0.001-1 microg/ml) dose-dependently releases histamine from mast cell at physiological extracellular Na' (134 mM) and Ca2+ (1mM) conditions. Lowering extracellular Ca2+ ions to 0.1 mM reduced histamine response to nearly basal level. However, the removal of extracellular Na+ ions significantly enhanced the secretion provoked by NGF (0.6 microg/ml) in low Ca2+ medium. Amiloride, an inhibitor of Na+/Ca2+ and Na+/H+ exchangers inhibited the potentiating effect of sodium free conditions. Conclusions: Our results suggest that the activity of Na+/Ca2+ and/or Na+/H+ exchange mechanisms could be of particular importance in the secretory process of mast cells induced by NGF.
Descriptors		MAST CELLS SODIUM CALCIUM NERVE GROWTH FACTORS HISTAMINE RELEASE RATS, WISTAR AMILORIDE