Author/Editor		Nemeth, L; Štiblar-Martinčič, D
Title		Kvantitativna analiza odziva zobne pulpe na preparacijo in jedkanje kavitete pri podganah
Translated title		Quantitative analysis of dental pulp response to cavity and acid etching in rats
Type		članek
Source		Zobozdrav Vestn
Vol. and No.		Letnik 58, št. 2
Publication year		2003
Volume		str. 42-9
Language		slo
Abstract		Background: Our previous qualitative analysis of the dental pulp response in etched and unetched cavity revealed that the changes were similar in quality but differed in degree. For this reason we wanted to analyse the alterations quantitatively. Materials and Methods: The same animals and operative procedures were used as in the previous qualitative and semiquantitative analysis. Following the sacrifice of animals 5 min, 6 h, 24 h and 72 h postoperatively, histological sections of teeth, stained with HE, Giemsa, Goldner, and Brown & Brenn methods, were analysed morphometrically. We determined the number of odontoblasts beneath the cavity and the number of aspirated cells in dentinal tubules, the predentin width, and the number of bacterial colonies in the cavity and dentinal tubules. Results: Acid etching caused an additional reduction in the number of odontoblasts and increase in the number of aspirated cells beneath the cavity 5 min, 6 h and 24 h postoperatively, but an increased number of odontoblasts 72 hours postoperatively, compared to unetched cavities. The predentin width was reduced after both procedures. Seventy-two hours postoperatively normal dentinogenesis was evident under unetched cavities. Fewer bacterial colonies were present in the cavity and dentinal tubules after etching then when etching had been omitted. Conclusion: Odontoblast injury is more extensive after cavity preparation with acid etching compared to cavity preparation without acid etching. Etching stimulates odontoblast renewal, but the reparative dentin synthesis begins earlier beneath unetched cavities. Further study is needed to evaluate the dental pulp response to acid etching.
Summary		Izhodišče: V naši predhodni kvalitativni in polkvantitativni analizi odziva zobne pulpe pri jedkani in nejedkani kaviteti smo ugotovili podobne odzivne spremembe, ki pa so se razlikovale po izraženosti. To je bil razlog, da smo želeli te spremembe analizirati tudi kvantitativno. Materiali in metode: Raziskavo smo opravili na istih živalih in z enakimi operativnimi postopki, kot smo jih uporabili pri kvalitativni in polkvantitativni raziskavi. Živali smo žrtvovali 5 minut, 6 ur, 24 ur in 72 ur po posegu, histološke rezine zob smo pripravili z barvanji po metodah HE in Giemsi, Goldner ter po metodi Brown & Brenn in jih morfometrično analizirali. Določali smo število odontoblastov pod kaviteto in število aspiriranih celic v dentinskih kanalih, širino predentina in število bakterijskih kolonij v kaviteti in dentinskih kanalih. Rezultati: Jedkanje kavitete je povzročilo dodatno zmanjšanje števila odontoblastov in povečanje števila aspiriranih celic pod kaviteto 5 minut, 6 ur in 24 ur po posegu; 72 ur po posegu se je število odontoblastov povečalo v primerjavi z nejedkano kaviteto. Širina predentina se je zmanjšala po obeh posegih, po 72-ih urah je bila dentinogeneza pod nejedkano kaviteto normalna. V jedkani kaviteti in dentinskih kanalih je bilo manj bakterij kakor pri nejedkani. Zaključek: Poškodba odontoblastov je po preparaciji in jedkanju kavitete večja kakor samo po preparaciji kavitete. Jedkanje spodbudi obnovo odontoblastov, vendar se tvorba reparativnega dentina prične prej pri nejedkani kaviteti. Za dokončno oceno odziva zobne pulpe na jedkanje kavitete bi bilo potrebno raziskavo podaljšati.
Descriptors		DENTAL PULP TEST ACID ETCHING, DENTAL DENTAL PULP ODONTOBLASTS RATS DENTAL CAVITY PREPARATION BACTERIA CELL COUNT