Avtor/Urednik		Repnik, Urška; Knežević, Miomir; Jeras, MAtjaž
Naslov		Simple and cost-effective isolation of monocytes from buffy coats
Tip		članek
Vir		J Immunol Methods
Vol. in št.		Letnik 278, št. 1-2
Leto izdaje		2003
Obseg		str. 283-92
Jezik		eng
Abstrakt		We have optimized the procedure of monocyte isolation on a Percoll density gradient. The new procedure consists of three steps: (1) the isolation of MNC on a Ficoll density gradient; (2) the separation of monocytes from lymphocytes on a high-density hyper-osmotic Percoll density gradient; and (3) the separation of monocytes from platelets and dead cells on a low-density iso-osmotic Percoll density gradient. The procedure is simple and cost-effective. Monocyte purity and recovery are both about 75% and platelet contamination is low. The isolated monocytes retain their capacity to differentiate into dendritic cells in vitro.
Deskriptorji		MONOCYTES CELL COUNT LEUKOCYTES, MONONUCLEAR BLOOD PLATELETS FLOW CYTOMETRY PHENOTYPE COST-BENEFIT ANALYSIS