Avtor/Urednik		Gligorievska, Nada
Naslov		Preživetje, migracija in diferenciacija človeških stromalnih celic iz kostnega mozga (SCKM) po presaditvi v podganje centralno živčevje
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2003
Obseg		str. 64
Jezik		slo
Abstrakt		Stem cell research offers possibilities for development of alternative types of therapy for neurodegenerative disorders. This class of disorders is characterized by degeneration of a group of neurons due to currently unknown reasons. Stem cells are those cells that have the abilities for self-renewal and differentiation into different cell types with specialized function. They can be found in bone marrow, blood (placentar and peripheral), eyes, brains, skeletal muscles, dental pulp, liver, skin, digestive epithelium, pancreas and umbilical cord. In our study, we used human bone marrow stromal cells (BMSC). These cells are easily accessible, have large proliferative potential in vitro, and are multipotent. The hypothesis of our study was that BMSC are capable of survival, migration and differentiation into neural cells, after transplantation into intact central nervous system of rats. That is why we have followed their survival, migration and differentiation after transplantation into the rat,central nervous system. We grew BMSC in vitro, labeled them and then stereotactically transplanted them into the rat striata. The prelabeling in vitro was used for evaluation of the cells' survival and migration. We used immunohistochemical and immunofluorescent labeling of specific antigens to detect potential differentiation of BMSC and subsequently estimated spatial colocalization of the same antigens and the labeled nuclei of the transplanted cells. By these means, we have tested if the transplanted cells expressed antigen characteristics of astrocytes, neurons, oligodendrocytes or microglia. We found out that, 28 days after transplantion into recipient brains, numerous BMSC could be found (minimal average number of cells was 9237`3908 i.e 30%). These cells have migrated into different areas of the recepient brain (up to 720 pm from the transplant), but most of them did not express antigens specific for neural cells. (Abstract truncated at 2000 characters).
Deskriptorji		BONE MARROW TRANSPLANTATION CORPUS STRIATUM STROMAL CELLS CELL SURVIVAL CELL DIFFERENTIATION RATS, WISTAR FLUORESCENT ANTIBODY TECHNIQUE IMMUNOHISTOCHEMISTRY IMAGE PROCESSING, COMPUTER-ASSISTED FLOW CYTOMETRY CELL COUNT