Avtor/Urednik		Avšič-Županc, T
Naslov		Antigenske lastnosti hantavirusa Dobrava in njegova patogenost
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		1991
Obseg		str. 76 (149)
Jezik		slo
Abstrakt		Hemorrhagic Fever with Renal Syndrome (HFRS), caused by hantaviruses, is also present in Slovenia. To clarify the epizootiology of hantavirus infection, small mammals captured in natural foci of HFRS were studied. Data indicate a substantial evidence that the bank vole, Clethrionomys glareolus, and yellow-necked field mice, Apodemus flavicollis are the main hosts for hantaviruses in endemic areas in Slovenia. A hantavirus was isolated from lungs of an A. flavicollis captured in Dobrava village. In addition, two more hantavirus strains has been isolated from wild rodents and one from a fatal HFRS case. The isolate from A. flavicollis, designated Dobrava, was antigenicaly compared with viruses representative of each of known serogroups within the hantavirus genus. Based upon serological reactivity of monoclonal antibodies it was established that antigenic properties of Dobrava virus are different from other hantaviruses. RNA from all new isolates were reverse transcribed to cDNA and successfully amplified by polimerase chain reaction (PCR). The amplified products were digested with restriction endonucleases. Analyses of the restriction fragments indicate that Dobrava virus has a unique restriction pattern, therefore represent a new hantavirus type. The involvence of Dobrava virus in HFSR was studied by demonstrating specific antibodies in the serea from HFSR patients. It revealed that antibodies against Dobrava virus are present in HFSR patients. We presume that Dobrava virus is not the etiologic agent that causes severe forms of HFSR in Slovenia.
Deskriptorji		MURIDAE HEMORRHAGIC FEVER VIRUS, EPIDEMIC SEROTYPING HEMORRHAGIC FEVER, EPIDEMIC SERODIAGNOSIS VIRUS CULTIVATION IMMUNOENZYME TECHNIQUES VIRION RNA, VIRAL OLIGONUKLEOTIDE PROBES SLOVENIA ANTIBODIES, MONOCLONAL IMMUNE SERA FLUORESCENT ANTIBODY TECHNIQUE