Avtor/Urednik		Jezernik, Kristijan; Romih, Rok; Mannherz, Hans Georg; Koprivec, Daša
Naslov		Immunohistochemical detection of apoptosis, proliferation and inducible nitric oxide synthase in rat urothelium damaged by cyclophosphamide treatment
Tip		članek
Vir		Cell Biol Int
Vol. in št.		Letnik 27
Leto izdaje		2003
Obseg		str. 863-9
Jezik		eng
Abstrakt		The present study was conducted to investigate cell death, proliferation and inducible nitric oxide synthase (iNOS) immunoreactivity in rat urothelium within 24 h after a single intraperitoneal dose of cyclophosphamide (CP). Necrotic cells were identified predominantly in the superficial cell layer from 1 h until 6 h after CP injection, most of them desquamating from the urothelium into the lumen of the urinary bladder. Active caspase-3 immunohistochemistry revealed apoptotic cells from 12 h until 24 h after CP injection. The apoptotic index reached a peak at 18 h and then rapidly dropped. Simultaneously with the decrease of apoptosis, the proliferation index increased from 18 h until 24 h after CP treatment. Immunoreaction to iNOS was first detected at 6 h in basal and intermediate cells. Later, iNOS immunoreactivity became stronger and was present in all cell layers. Our results suggest that the destruction of rat urothelium during 24 h after CP administration is due not only to necrosis, but also to apoptosis. The first 6 h are characterised by necrotic changes and no iNOS immunoreactivity. Thereafter, apoptosis and iNOS immunoreactivity are observed within the damaged urothelium. At 24 h after CP injection, iNOS immunoreactivity is still present, but proliferation prevails over cell death, enabling the urothelium to start regeneration.
Deskriptorji		BLADDER APOPTOSIS UROTHELIUM CELL DIVISION NITRIC-OXIDE SYNTHASE RATS, INBRED F344 CYCLOPHOSPHAMIDE MICROSCOPY, ELECTRON PROLIFERATING CELL NUCLEAR ANTIGEN IMMUNOHISTOCHEMISTRY