Avtor/Urednik		Joksimović, Čedomir
Naslov		Izražanje kaspaze-3 in survivina v hepatocelularnem karcinomu in v netumorskih jetrih ob tumorju
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2003
Obseg		str. 58
Jezik		slo
Abstrakt		Apoptosis or programmed cell death is an orderly cascade that ultimately results in demise of cells and therefore preserves the balance between cell proliferation and cell death. This is of great importance for normal functioning of the human body. Many studies have shown that disregulation of apoptosis is an important factor in progression of hepatocellular carcinoma (HCC) - one of the most common cancers worldwide. The percentage of apoptotic cells in HCC, compared to non-tumorous liver tissue was investigated. The role of caspase-3, which is an important apoptotic protein, was of great interest for this study. Expression of unprocessed, as well as activated form of caspase-3, was analysed and the expression of survivin, an inhibitor of apoptosis, was investigated. Twenty patients with HCC were included in the study. Two samples were obtained from each patient, one of HCC and one of non-tumorous liver. TUNEL and immunohistochemical staining for the detection of activated form of caspase-3 were used to determine the percentage of apoptotic cells (apoptotic index). Immunohistochemical staining was also performed for detection of procaspase-3 and survivin expression. Apoptotic index (AI) determined by TUNEL as well as by immunohistochemical staining for the detection of active form of caspase-3 was higher in HCC (0,55 t 0,477 and 1,532 t 1,05, respectively) compared to non-tumorous liver (0,44% ± 0,485 and 0,426 ± 0,62, respectively). Both indices were also higher in less differentiated HCCs. Positive staining for procaspase-3 was present in 75% of HCC. Positive correlation between expression of procaspase-3 and activated form of caspase-3 proved to be statistically significant. Survivin positive reaction was detected in 70% of HCC. Stronger expression of survivin was detected in HCC compared to non-tumorous liver. (Abstract truncated at 2000 characters).
Deskriptorji		LIVER NEOPLASMS CARCINOMA, HEPATOCELLULAR APOPTOSIS CYSTEINE PROTEINASES IMMUNOHISTOCHEMISTRY DNA FRAGMENTATION NEOPLASM STAGING CELL COUNT GENE EXPRESSION