Avtor/Urednik		Zrimšek, Petra; Kosec, M; Mrkun, J
Naslov		Redukcijski test z resazurinom za ugotavljanje kvalitete semenčic
Prevedeni naslov		Resazurin reduction test to evaluate semen quality
Tip		članek
Vir		Vet Nov
Vol. in št.		Letnik 29, št. 4
Leto izdaje		2003
Obseg		str. 183-6
Jezik		slo
Abstrakt		The resazurin reduction test depends on the ability of metabolically active spermatozoa to reduce the resazurin redox dye to resorufin which manifested as visual colour change from blue (resazurin) to pink (resorufin) and further to white (dihydroresorufin). The presented test is a simple, reliable and reproducible method for the evaluation of semen samples. The colour change of resazurin is matched with colour chart, consisting of a spectrum of colours from blue to pink, but varied among investigators. However, there may be a possibility of human error in assessing the quality of samples, as the method is based on the visual comparison of the colours. Therefore, spectrophotometric application of resazurin reduction test has recently been preferable. In the present article the use of resazurin reduction test in evaluation of human, stallion, bull, ram and boar semen is disscused.
Izvleček		Redukcijski test z resazurinom temelji na sposobnosti metabolično aktivnih semenčic reducirati redoks barvilo resazurin v resorufin. Redukcija barvila se kaže v spremembi barve iz modre (resazurin) v roza (resorufin) in naprej v belo (dihidroresorufin). Omenjeni test za določanje kvalitete semena je enostaven, zanesljiv in ponovljiv. Spremembo barve resazurina lahko določamo na podlagi barvne skale, ki si jo pripravimo sami in je sestavljena iz barvne lestvice od modre do roza, vendar pa pri tem zaradi subjektivnih ocen analitika lahko pride do napak. V zadnjem častc se zato vse več uporablja spektrofotometrično določevanje produkta reakcije. V članku so predstavljene različne aplikacije redukcijskega testa z resaztcrinom za ugotavljanje kvalitete humanega, žrebčevega, bikovega, ovnovega in merjaščevega semena.
Deskriptorji		SEMEN SPERMATOZOA SPERM MOTILITY REDUCING AGENTS