Avtor/Urednik		Flegel, WA; Čurin-Šerbec, V; Delamaire, M; Donvito, B; Ikeda, H; Jorgensen, J; Kumpel, B; Le-Pennec, P-Y; Pisačka, M; Tani, Y
Naslov		Section 1B: Rh flow cytometry. Coordinator's report. Rhesus index and antigen density: an analysis of the reproducibility of flow cytometric determination
Tip		članek
Vir		Transfus Clin Biol
Vol. in št.		Letnik 9
Leto izdaje		2002
Obseg		str. 33-42
Jezik		eng
Abstrakt		Fifty-seven IgG monoclonal anti-D antibodies were evaluated in the Rh flow cytometry section, in which 12 laboratories participated. Staining protocols and a fluorescein (FITC)-conjugated Fab fragment goat anti-human IgG (H+ L) as a secondary antibody were recommended but not mandatory. A CcDEe red blood cell (RBC) sample that was shown to be homorygous forRHD by molecular methods was supplied and used as internal 'standard RBC' throughout all experiments. An RBC panel comprising two partial D and four weak D types was supplied as well. The use of standard RBC reduced the variability of the data among the laboratories and allowed the conversion of fluorescence data into epitope densities, which were compounded in an antigen density (antigen D per RBC). The highest antigen denšdy was determined for DVI type III, followed by DVII and weak D type 3; the lowest antigen density were determined for weak D type 1 and type 2. Nine of the 12 participating laboratories discriminated three groups of aberrant RhD that had similar Rhesus indices (RI): D category VI with RI = 0; weak D type 2 and type 3 with an high RI; and D category VII and weak D type 1 with an intermediate RI. The antigen densities and the Rhesus indices obtained correlated well among the laboratories of this Workshop section despite different staining protocols, secondary antibodies and instrumentation.
Deskriptorji		ANTIBODIES, MONOCLONAL FLOW CYTOMETRY ISOANTIBODIES RH-HR BLOOD-GROUP SYSTEM ALGORITHMS DATA DISPLAY EPITOPES ERYTHROCYTE MEMBRANE FLUORESCENT DYES FLUOROMETRY GOATS IMMUNOGLOBULINS, FAB REFERENCE STANDARDS REPRODUCIBILITY OF RESULTS SPECIMEN HANDLING