Avtor/Urednik		Podgornik, Helena; Podgornik, Aleš
Naslov		Separation of manganese peroxidase isoenzymes on strong anion-exchange monolithic column using pH-salt gradient
Tip		članek
Vir		J Chromatogr B
Vol. in št.		Letnik 799
Leto izdaje		2004
Obseg		str. 343-7
Jezik		eng
Abstrakt		Different chromatographic methods including chromatofocusing are used for separation of manganese peroxidase (MnP) isoforms and their isolation from the fungal growth medium. We tested strong anion exchange methacrylate based monolithic columns as a stationary phase for fast separation of MnP's. Sodium acetate buffers of two different pH values (6 and 4) were used for formation of reproducible pH gradient. The entire cycle, involving analysis and column regeneration, was completed in 3 min. Use of pH gradient showed better MnP isoform separation comparing to the salt gradient, while application of combined pH-salt gradient, resulted in further improvement
Deskriptorji		CHRYSOSPORIUM ISOENZYMES MANGANESE PEROXIDASES HYDROGEN-ION CONCENTRATION CHROMATOGRAPHY