Avtor/Urednik		Stibilj, V; Mazej, D; Falnoga, I
Naslov		A study of low level selenium determination by hybride generation atomic fluorescence spectrometry in water soluble protein and peptide fractions
Tip		članek
Vir		Analytical and bioanalytical chemistry
Vol. in št.		Letnik 377
Leto izdaje		2003
Obseg		str. 1175-83
Jezik		eng
Abstrakt		Development of a method for very low level selenium determination in water soluble protein and peptide fractions, obtained after various separation procedures, is presented. A hydride generation atomic fluorescence spectrometry (HG-AFS) detection system was otimised and the influence of Cu(II), Sb(V), As(III) and HNO3 interferences in the measurement of Se by HG-AFS was investigated. A destruction procedure using HN3 and H2O2 was also optimised and the average recovery of the digestion of a solution of selenometioneine was 92 +- 4% (n=14). Combination of this digestion system gave reliable results. Accuracy was tested by comparison with two independent methods. A very low detection limit (DL) of 0.2 ng/g of measuring solution was achieved. Rhe whole procedure form weighing to measuring was performed in the samr Teflone tube. The addition of HNO3 to the fractions before long term storage at -20C was neccessary to prevent adsorbtion on the test tubes. Selenium was measured in water soluble protein and peptide fractions obtained after extraction, and Sephadex G-75 chromatography performed on liver samples from: i) hens exposed to As2O3, ii) hens fed with a high fat feed and the certified reference material dogfish liver (CRM DOLT-2). Because of the very low DL we were able to observe the Se distribution in chromatographic fractions of samples of organisms which were not exposed to excess amounts of Se. The presence of selenium associated with metallothioneins was observed.
Deskriptorji		SELENIUM LIVER METALLOTHIONEIN POULTRY COPPER ANTIMONY ARSENIC NITRIC OXIDE SPECTROMETRY, FLUORESCENCE CHROMATOGRAPHY