Avtor/Urednik		Falnoga, Ingrid; Stibilj, Vekoslava; Tušek-Žnidarič, Majda; Šlejkovec, Zdenka; Mazej, Darja; Jaćimović, Radojko; Ščančar, Janez
Naslov		Effect of arsenic trioxide on metallothionein and its conversion to different arsenic metabolites in hen liver
Tip		članek
Vir		Biol Trace Elem Res
Vol. in št.		Letnik 78
Leto izdaje		2000
Obseg		str. 241-54
Jezik		eng
Abstrakt		The metabolism o arsenic, its affinity to metallothionein (MT), its influence on selenium levels, and its biotransformation to different metabolites in the liver tissue of laying hens exposed to arsenic trioxide (As2O3) was investigated. The experiment was performed with two groups of hens for 19 d with either a standard diet ot with the same diet enriched in arsenic (30 microg/g). The mayor findings were as follows: 1. After 19 d exposure, about 65% of the total liver As was found in the water-soluble phase (100,000g centrifuged supernatant). In liver supernatant, As binding was found mostly in the range of very low-molecular-weight proteins (Mr<10,000).Alhough after exposure he amount of MT-like proteins increased, the As bound to it was only in trace amounts. The protein was identified by convential procedures as Zn, Cu-thionein with traces of selenium and arsenic. 2. Arsenic exposure resulted in almost unchanged Se levels regarding its tissue concentrations and distribution between supernatant and pellet, where about 10% of total Se was found in the supernatant. On the contrary, As exposure did affect Cd levels. Tissue Cd concentration was slightly diminished, but the percentage of tissue Cd found in the water-soluble phase was increased from 20% to 40%. 3. In methanol extracts of tissue and supernatant of the As-exposed group, only two arsenic compounds were detected, As(III) and dimethylarsinic acid (DMA), the latter prevailing.
Deskriptorji		ARSENICALS METALLOTHIONEIN BIOTRANSFORMATION ARSENIC SELENIUM CADMIUM COPPER ZINC LIVER POULTRY NEUTRON ACTIVATION ANALYSIS SPECTROPHOTOMETRY, ATOMIC ABSORPTION CHROMATOGRAPHY, HIGH PRESSURE LIQUID