Avtor/Urednik		Vilfan, Tanja; Černila, Boštjan; Črešnar, Bronislava; Breskvar, Katja
Naslov		Identification of regulatory elements in stress inducible epimerase promoter from filamentous fungus Rhizopus nigricans
Tip		članek
Vir		Acta Chim Slov
Vol. in št.		Letnik 50
Leto izdaje		2003
Obseg		str. 751-6
Jezik		eng
Abstrakt		Our previous studies revealed that exposure of tilamentous fungus Rhizopus nigricans (R. nigricans) to different stressors up-regulates the expression of cytosolic hsp70 and epimerase genes. Analysis of the epimerase gene promoter showed that it includes putative binding sites for transcriptional activators and repressors, which could be involved in the control of gene expression. We proposed that two types of regulatory elements could regulate the epimerase gene induction during heat shock: two putative heat shock elements (HSE) and possibly STRE, present in many promoters of yeast Saccharomyces cerevisiae (S. cerevisiae) genes which are induced in different stress conditions, including heat shock. The aim of the present study was to investigate in vitro the two types of promoter regulatory elements, possibly involved in activation of the epimerase gene during heat shock. The results obtained with electrophoretic mobility shift assay (EMSA) showed that in the fungus R. nigricans the epimerase gene is induced during heat shock via HSF-independent mechanism and that heat shock activates transcription factors capable of binding to putative STRE regulatory element.
Izvleček		Predhodne raziskave so pokazale, da različni stresni pogoji v filamentozni glivi Rhizopus nigricans (R. nigricans) povečajo izražanje genov za citosolne Hsp70 in epimerazo. Z analizo epimeraznega promotorja smo ugotovili, da promotor vsebuje morebitna vezavna mesta za transkripcijske dejavnike, ki bi lahko uravnavali izražanje gena za epimerazo. Izražanje gena za epimerazo med toplotnim stresom bi bilo lahko uravnavano preko dveh vrst regulatornih elementov: dveh morebitnih elementov HSE in preko elementa STRE, ki je prisoten v števiInih promotorjih genov kvasovke Saccharomyces cerevisiae (S. cerevisiae) in se izražajo v različnih stresnih pogojih, vključno med toplotnim stresom. Cilj naše raziskave je bil proučiti obe vrsti regutatornih elementov in njihovo vlogo med toplotnim stresom v pogojih in vitro. Rezultati poskusov premikov kompteksov DNA proteini v električnem polju (EMSA) so pokazali, da je v glivi R. nigricans mehanizem povečanega izražanja gena za epimerazo neodvisen od dejavnikov HSF in da toplotni stres aktivira transkripcijske dejavnike, ki se vežejo na morebitni regulatorni element STRE.
Deskriptorji		HEAT-SHOCK PROTEINS 70 HEAT-SHOCK RESPONSE EPIMERASES PROMOTER REGIONS (GENETICS) ELECTROPHORESIS