Avtor/Urednik		Sajko, Špela; Kubinova, Lucie; Cvetko, Erika; Kreft, Marko; Wernig, Anton; Eržen, Ida
Naslov		Frequency of M-cadherin-stained satellite cells declines in human muscles during aging
Tip		članek
Vir		J Histochem Cytochem
Vol. in št.		Letnik 52, št. 2
Leto izdaje		2004
Obseg		str. 179-85
Jezik		eng
Abstrakt		SUMMARY To answer the question of whether the satellite cell pool in human muscle is reduced during aging, we detected satellite cells in 30-micro m-thick transverse sections under the confocal microscope by binding of M-cadherin antibody. The basal lamina was deteded with laminin. Nuclei were stained with bisbenzimide or propidium iodide. Satellite cells were counted by applying the disector method and unbiased sampling design. To determine if there are age-related differences in muscle fiber types, morphometric charaderistics of muscle fibers were examined on thin sections stained for myofibrillar ATPase. Autopsy samples of vastus lateralis muscle from six young (28.7 ± 2.3 years) and six old (70.8 ± 1.3 years) persons who had suffered sudden death were analyzed. Numbers of satellite cells per fiber length (NsdLfib) and number of satellite cells per total number of nuclei (satellite cell nuclei + myonuclei) (NsdNnucl) were significantly lower in the old group (p< 0.05). We demonstrate the importance of proper sampling and counting in estimation of sparsely distributed structures such as satellite cells. Our results support the hypothesis that the satellite cell fraction declines during aging.
Deskriptorji		MUSCLE, SKELETAL MUSCLE FIBERS CADHERINS LAMININ BASEMENT MEMBRANE ADULT AGED CELL NUCLEUS DESMIN SARCOMERES DEATH, SUDDEN AUTOPSY MYOFIBRILS MICROSCOPY, CONFOCAL ADENOSINETRIPHOSPHATASE IMMUNOHISTOCHEMISTRY