Avtor/Urednik		Glavan, Gordana
Naslov		Dopaminergično uravnavanje izražanja mRNK sinaptotagminov v striatumu podgan z enostransko poškodbo nigrostriatne poti
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2003
Obseg		str. 90
Jezik		slo
Abstrakt		In Parkinson's disease (PD), the progressive degeneration of nigrostriatal dopaminergic neurons results in a severe lack of dopamine in the striatum that leads in the changes of gene expression, involved in the disturbances of neurotransmission in striatum and its targets. Synaptotagmins (Syts) are involved in the release of classical neurotransmitters and neuropeptides. Therefore it's possible that the pathophysiological changes of neurotransmission in PD and side effects after chronic L-DOPA treatment are accompanied with the changes of the expression of syts mRNA in the striatum. The aim of this study was to investigate the expression of syts mRNA on rat model for PD and on the model for unwanted side effects (dyskinesias) that occur in PD after the stimulation of D1 receptors with L-DOPA. We hypothesized that: 1) Dopaminergic striatal denervation results in the changes in the expression of mRNA for syts in the striatum; 2) The acute stimulation of dopamine D1 receptors with SKF-82958 or L-DOPA induces the expression of syts mRNA in the denervated striatum; 3) The intermittent treatment 6-OHDA rats with L-DOPA enhances the inducibility syts mRNA in the denervated striatum. We used the rat model for PD. We injected a toxin 6-hydroxydopamine (6-OHDA) in the nigrostriatal pathway in order to selectively destroy nigrostriatal dopamine neurons on one side of the brain (hemiparkinsonian rat model). We treated acutely 6-OHDA animals with a selective dopamine D1 receptor agonist SKF-82958 and acutely/chronically with a dopamine precursor L-DOPA. We then tested the behavior of these animals. The levels of mRNA expression in the striatum of these animals were determined with radioactive in situ hybridization. The acute treatment with L-DOPA or SKF-82958 provoked a contralateral turning of 6OHDA rats a result of stimulation of dopamine Dl receptors. (Abstract truncated at 2000 characters).
Deskriptorji		PARKINSON DISEASE DYSKINESIA, DRUG-INDUCED CORPUS STRIATUM LEVODOPA RECEPTORS, DOPAMINE D1 OXIDOPAMINE BEHAVIOR, ANIMAL GLUTAMATE DECARBOXYLASE IN SITU HYBRIDIZATION OLIGONUCLEOTIDE PROBES DISEASE MODELS, ANIMAL TACHYKININS ENKEPHALINS SYNAPTIC VESICLES RATS