Avtor/Urednik		Kogej, Tina; Wheeler, Michael H; Lanišnik-Rižner, Tea; Gunde-Cimerman, Nina
Naslov		Evidence for 1,8-dihydroxynaphthalene melanin in three halophilic black yeasts grown under saline and non-saline conditions
Tip		članek
Vir		Fems Microbiol Lett
Vol. in št.		Letnik 232
Leto izdaje		2004
Obseg		str. 203-9
Jezik		eng
Abstrakt		The ascomycetous black yeasts Hortaea werneckii, Phaeotheca triangularis, aud Trimmatostroma salinum are halophilic fungi that inhabit hypersaline water of solar salterns. They are characterized by slow, meristematic growth and very thick, darkly pigmented cell walls. The dark pigment, generally thought to be melanin, is consistently present in their cell walls when they grow under saline and nonsaline conditions. We used the inhibitor tricyclazole to test the fungi in this study for the presence of 1,8-dihydroxynaphthalene (DHN)melanin biosynthesis, since fungal melanins reportedly are derived either from DHN, tyrosine via 3,4-dihydroxyphenylalauine, gamma-glutaminyl-3,4-dihydroxybenzene, or catechol. Tricyclazole-treated cultures of the fungi were reddish-brown in color and contained typical intermediates of the DHN-melanin pathway, as demonstrated by high-performance liquid chromatography. This investigation showed that the three fungi synthesized DHN-melanin under saline and non-saline growth conditions.
Deskriptorji		NAPHTHOLS MELANINS ASCOMYCETES SODIUM CHLORIDE CHROMATOGRAPHY, THIN LAYER CHROMATOGRAPHY, HIGH PRESSURE LIQUID