Avtor/Urednik		Černila, Boštjan; Črešnar, Bronislava; Breskvar, Katja
Naslov		Molecular characterization of genes encoding cytosolic Hsp70s in the zygomycete fungus Rhizopus nigricans
Tip		članek
Vir		Cell Stress Chaperones
Vol. in št.		Letnik 8, št. 4
Leto izdaje		2003
Obseg		str. 317-28
Jezik		eng
Abstrakt		Previous studies have shown that some stressors, including steroid hormones 21-OH progesterone and testosterone, stimulate the accumulation of heat shock protein 70 (hsp70) messenger ribonucleic acid (mRNA) population in the zygomycete filamentous fungus Rhizopus nigricans. In this study we report the cloning of 3 R nigricans hsp70 genes (Rnhsp70-1, Rnhsp70-2, and Rnhsp70-3) encoding cytosolic Hsp70s. With a Southern blot experiment under high stringency conditions we did not detect any additional highly homologous copies of the cytosolic hsp70 genes in the R nigricans genome. Sequence analyses showed that alI 3 genes contain introns within the open reading frame. The dynamics of the R nigricans molecular response to progesterone, 21-OH progesterone, and testosterone, as well as to heat shock, copper ions, hydrogen peroxide, and ethanol was studied by temporal analysis of Rnhsp70-i and Rnhsp70-2 mRNA accumulation. Northern blot experiments revealed that the Rnhsp70-2 transcript level Is not affected by testosterone, whereas mRNA levels of both genes are rapidly increased with all the other stressors studied. Moreover, the decrease of transcript levels is notably delayed in ethanol stress, and a difference is observed between the profiles of Rnhsp70-i and Rnhsp70-2 transcripts during heat stress.
Deskriptorji		RHIZOPUS HEAT-SHOCK PROTEINS 70 GENOME, FUNGAL GENOMIC LIBRARY CLONING, MOLECULAR DNA, COMPLEMENTARY TESTOSTERONE DESOXYCORTICOSTERONE PROGESTERONE CYTOSOL INTRONS RNA, MESSENGER BLOTTING, NORTHERN BLOTTING, SOUTHERN