Avtor/Urednik		Rupnik, M; Grabnar, M; Gerič, B
Naslov		Binary toxin producing Clostridium difficile strains
Tip		članek
Vir		Anaerobe
Vol. in št.		Letnik 9
Leto izdaje		2003
Obseg		str. 289-94
Jezik		eng
Abstrakt		Clostridium difficile produces three toxins, TcdA, TcdB and CDT. TcdA and TcdB are single-stranded molecules acting as glucosyltransferases specific for small GTPases. CDT is an actin specific ADP-ribosylating binary toxin characteristically composed of two independent components, enzymatic CDTa (48 kDa) and binding CDTb (99 kDa). The cdtA and cdtB genes were sequenced in two CDT-positive strains of C. difficile (CD 196 and 8864) and at least two CDT-negative strains with truncated form of binary toxin gencs are known (VPI 10463 and C difficile genome strain 630). The prevalence of binary toxin producing strains is estimated to be from 1.6% to 5.5%, although a much higher proportion has been reported in some studies. The role of the binary toxin as an additional virulence factor is discussed.
Deskriptorji		CLOSTRIDIUM DIFFICILE VIRULENCE BACTERIAL TOXINS GENOME, BACTERIAL POLYMERASE CHAIN REACTION