Avtor/Urednik		Lenasi, Valentina
Naslov		Uravnavanje izražanja kazeinskih genov pri konju (Equus caballus)
Prevedeni naslov		Regulation of the equine casein genes expression
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2004
Obseg		str. 119
Jezik		slo
Abstrakt		As a main protein fraction in milk, caseins are the most important source of amino acids for the suckling infant. Expression of casein genes is complex, tissue specific and precisely regulated process. Due to high similarities between the human and mare milk's composition, mare's milk could be used as a healthier alternative to cow's milk, especially for people with allergies to cow milk proteins. As little is known about the molecular basis of equine casein synthesis, we aimed to investigate the regulation of casein gene expression at the level of transcription as well as at the level of mRNA splicing. We searched for positive and negative cis-regulating elements in beta- and K-casein proximal promoter region. Using electro mobility shift assay (EMSA), we examined the functionality of transcription activator STATS binding sites in both promoters. We induced transcription of a reporter gene that was under control of `- and K- casein promoters by lactogenic hormones and compared their efficiency of induction. In the case of beta-casein promoter, we also tested the effect of promoter length on transcription activation. Induction of gene expression by lactogenic hormones was more efficient under the control of the K-casein promoter (1887 bp) compared to the beta-casein promoter. The longer beta-casein promoter variant (1920 bp) induced weaker expression than the shorter one (828 bp), however, using the shorter variant (828 bp) together with the part of intron 1 (435 bp) yielded a very low expression level. Two alternatively spliced exons were found in the asl- (exons 8 and 15) as well as in the beta-casein mRNA (exons 5 and 8). The analysis of alpha s1-casein mRNA expression revealed the same pattern of inclusion of weak exons in the samples of all tested animals. In contrast, the beta-casein mRNA showed three different splicing patterns of inclusion of weak exons. (Abstract truncated at 2000 characters).
Deskriptorji		CASEINS LACTATION TRANSFECTION PROMOTER REGIONS (GENETICS) GENE EXPRESSION TRANSCRIPTION, GENETIC HORSES BASE SEQUENCE ELECTROPHORESIS, AGAR GEL CLONING, MOLECULAR LUCIFERASE BETA-GALACTOSIDASE CELL LINE