Avtor/Urednik		Caserman, Simon
Naslov		Vloga proteoliznih encimov pri invazivni rasti endotelijskih celic med procesom angiogeneze
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Univerza v Ljubljani, Medicinska fakulteta
Leto izdaje		2004
Obseg		str. 112
Jezik		slo
Abstrakt		Angiogenesis is a process of development of new capillaries from pre-existing blood vessels. In adults it is involved in different physiologic in pathologic tissue remodelling. Angiogenesis is also one of the crucial events in tumour progression and metastasis, hence being the subject of extensive scientific efforts to develop antiangiogenic approaches in treatment of cancer. Specific targeting of tumour blood vessels is based on differences between angiogenic and steady state endothelium. One of those differences is the elevation of proteolytic enzymes expression, contributing to invasive phenotype of endothelial cells in angiogenesis, similar to the development of malignant tumour cells. The major triggers of the shift from steady state to angiogenic endothelium in tumours are specific growth factors and proteins of extracellular matrix. In this study, we used aorta ring culture in collagen gel as a model of invasive capillary growth. The model resembles angiogenesis in vivo in several features. The morphology of new endothelial cell structures mimics morphology of native capillaries, capillary growth correlates with concentration of proangiogenic growth factors in the culture and capillary growth trough collagen matrix depends on proteolysis of the matrix. The phase of capillary growth is followed by capillary regression phase associated with collagen degradation in contact with aorta explant. We imitated capillary growth from aorta with primary culture of dermal endothelial cells to demonstrate selective influence of proangiogenic growth factors and collagen matrix on the induction of certain types of proteinases and their endogenous inhibitors that are known to be associated with cancer cell invasion. (Abstract truncated at 2000 characters).
Deskriptorji		NEOPLASMS ENDOTHELIUM, VASCULAR AORTA CAPILLARIES ANGIOGENESIS FACTOR ENDOTHELIUM METALLOPROTEINASES RNA, MESSENGER POLYMERASE CHAIN REACTION ENDOTHELIAL GROWTH FACTORS CELLS, CULTURED PLASMINOGEN ACTIVATORS CYSTEINE PROTEINASE INHIBITORS PLASMINOGEN ACTIVATOR INHIBITOR 1 COLLAGEN IMMUNOCHEMISTRY BASE SEQUENCE DISEASE MODELS, ANIMAL RATS