Avtor/Urednik		Odar, Katarina
Naslov		Preživetje celic pozobnice po izbitju zob
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2003
Obseg		str. 55
Jezik		slo
Abstrakt		BACKGROUND. Tooth avulsion is one of the most severe injuries to the teeth. The treatment of choice is replantation and immobilisation of the avulsed tooth. Prior to replantation, the teeth are often being stored in various manners. Periodontal ligament (PDL) cell viability is one of the most crucial factors affecting the prognosis of avulsed teeth. The higher the survival rate of the PDL cells during the time delay between avulsion and replantation, the lower the risk of root resorption and tooth loss. The PDL of the surgically extracted teeth provides us an easily accessible experimental model to study the PDL of the avulsed teeth. AIM OF THE STUDY. To objectively determine the time - dependence of PDL cell viability after the avulsion and to compare it between the two types of storage conditions (with and without the presence of physiologic saline solution). We also wanted to determine the impact of the patient's age on the PDL cell viability. We wanted to make a contribution for a more successful treatment planning. HYPOTHESIS. The percentage of the nonviable PDL cells increases with time after the tooth removal (storage time) and follows a certain time-curve. The storage conditions after the avulsion can affect the PDL cell survival of the stored teeth. The age of the patient has an important impact on the PDL cell survival. METHODS. The method we used has never been used for this purpose before. Surgically extracted human third molars were stored in tightly closed plastic containers (5 ml). To the first group of teeth (n = 64), physiologic saline solution was added, the second group (n = 41) was left dry. After various times ranging from 0 to 240 minutes, the PDL samples were obtained from the root surfaces and the cell viability was determined by using a nonvital cell staining with propidium iodide and total cell counting in a flow cytometer. (Abstract truncated at 2000 characters).
Deskriptorji		TOOTH AVULSION TOOTH REPLANTATION PERIODONTAL LIGAMENT CELL SURVIVAL TOOTH EXTRACTION MOLAR, THIRD FLOW CYTOMETRY TIME FACTORS