Avtor/Urednik		Hajdinjak, Tine; Zagradišnik, Boris
Naslov		Prostate cancer and polymorphism D85Y in gene for dihydrotestosterone degrading enzyme UGT2BI5: frequency of DD homozygotes increases with Gleason score
Tip		članek
Vir		Prostate
Vol. in št.		Letnik 59, št. 4
Leto izdaje		2004
Obseg		str. 436-9
Jezik		eng
Abstrakt		BACKGROUND. Although, a functional rationale for influence of polymorphism D85Y in gene UGT2B15 on prostate cancer (PCa) exists (different Vmax of enzyme), conflicting results have been reported. METHODS. DNA from 178 controls and 206 PCa patients with known Gleason score were genotyped using a newly developed RFLP assay, which allowed the detection of both alleles in an individual after single PCR amplification. RESULTS. Controls:16% DD, 52% DY; PCa patients: 23% DD, 49% DY. Subgroups of PCa: well differentiated: 11% DD, 37% DY; moderately differentiated: 22% DD, 50% DY; poorly differentiated: 34% DD, 50% DY. Correlation was confirmed between Gleason score and number of D alleles (P = 0.018) and persisted after age adjustment. When comparing controls to patients with a Gleason score of 7 or more, difference for the frequency of homozygosity DD was significant between the groups (P = 0.032, OR = 2.04). CONCLUSIONS. Polymorphism D85Y in gene UGT2B15 correlates with differentiation of PCa.
Deskriptorji		PROSTATIC NEOPLASMS POLYMORPHISM (GENETICS) GLUCURONOSYLTRANSFERASE GENOTYPE POLYMERASE CHAIN REACTION CASE-CONTROL STUDIES