Avtor/Urednik		Lanišnik-Rižner, Tea
Naslov		Konstitutivna 17beta-hidroksisteroidna dehidrogenaza pri glivah
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Fakulteta za narovoslovje in tehnologijo
Leto izdaje		1993
Obseg		str. 64
Jezik		slo
Abstrakt		The occurrence of a constitutive 17beta-hydroxysteroid dehydrogenase in fungi was investigated. Two representatives of four subphylum were tested: Mucor spinosus, Rhizopus nigricans (Zygomycotina); Pleospora herbarum, Cochliobolus lunatus (Ascomycotina); Fusarium 1ini, Trichoderma viride (Deuteromycotina); Pleurotus ostreatus and Stropharia rugosa (Basidiomycotina). It was found that the constitutive 17beta-hydroxysteroid dehyrogenase in the fungus Cochliobolus Iunatus ( which A. Plemenitaš et al. found in 1988) isn't unique, on the contrary, this enzyme seems to be common among fungi. 17beta-hydroxysteroid dehyrogenase is a regiospecific enzyme, active only on 17beta site of the steroid ring of substrates: androstendione, testosterone, estrone and 17beta-estradiol. In addition to the 17beta-hydroxysteroid dehyrogenase 20beta-HSDH activity was found in the fungus Trichoderma viride. This activity can be the result of one polyfunctional enzyme or two regioselective enzymes. The relationship between the phase of growth (age of fungi) and 17beta-hydroxysteroid dehyrogenase activity in fungi was studied. In most fungi the enzyme activity increased until the onset of the stationary phase of growth. In the stationary phase the activity remained constant or decreased. Beside that the subcelular localization of 17beta-hydroxysteroid dehyrogenase was tested. This enzyme was in five cases found in cytosol.
Deskriptorji		17-HYDROXYSTEROID DEHYDROGENASES BASIDIOMYCETES ZYGOMYCOTINA ASCOMYCETES DEUTEROMYCETES CHROMATOGRAPHY, THIN LAYER SPECTRUM ANALYSIS, MASS