Avtor/Urednik | Lipnik-Štangelj, M; Čarman-Kržan, M | |
Naslov | Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes | |
Tip | članek | |
Vir | Inflamm Res | |
Vol. in št. | Letnik 53, št. 6 | |
Leto izdaje | 2004 | |
Obseg | str. 245-52 | |
Jezik | eng | |
Abstrakt | Objective: Histamine stimulates nerve growth factor (NGF) secretion from cultured astrocytes. Histamine H1-receptor antagonists completely block its effect. In the present study, we determined the involvement of histamine-receptor subtypes in this process. Materials and methods: Radioligand-binding assay was used to establish the presence of histamine H1 and H2 receptors on new-born rat cortical astrocytes in primary culture. Histamine H… and H… receptor ligands, and highly selective protein kinase C (PKC) inhibitor were used to influence NGF secretion from cultured astrocytes. NGF, released into the culture medium, was measured by NGF-ELISA. Results: Histamine H4-receptor agonists (histamine, selected histaprodifens) increased the secretion of NGF from cultured astrocytes in a concentration-dependent manner. H4-receptor antagonists/inverse agonists (mepyramine, triprolidine) and PKC inhibitor completely blocked the effect of histamine. Histamine H2 and H4 receptor agonists did not enhance NGF secretion significantly. In addition, H2 and H3/H4 receptor antagonists did not diminish histamine-stimulated NGF release. Conclusions: Our results indicate that histamine H4 receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes. | |
Deskriptorji | ASTROCYTES NERVE GROWTH FACTORS RECEPTORS, HISTAMINE H1 RECEPTORS, HISTAMINE H2 RECEPTORS, HISTAMINE H3 RATS, WISTAR CELLS, CULTURED RADIOLIGAND ASSAY PROTEIN KINASE C HISTAMINE ENZYME-LINKED IMMUNOSORBENT ASSAY |