Avtor/Urednik     Lipnik-Štangelj, M; Čarman-Kržan, M
Naslov     Activation of histamine H1-receptor enhances neurotrophic factor secretion from cultured astrocytes
Tip     članek
Vir     Inflamm Res
Vol. in št.     Letnik 53, št. 6
Leto izdaje     2004
Obseg     str. 245-52
Jezik     eng
Abstrakt     Objective: Histamine stimulates nerve growth factor (NGF) secretion from cultured astrocytes. Histamine H1-receptor antagonists completely block its effect. In the present study, we determined the involvement of histamine-receptor subtypes in this process. Materials and methods: Radioligand-binding assay was used to establish the presence of histamine H1 and H2 receptors on new-born rat cortical astrocytes in primary culture. Histamine H… and H… receptor ligands, and highly selective protein kinase C (PKC) inhibitor were used to influence NGF secretion from cultured astrocytes. NGF, released into the culture medium, was measured by NGF-ELISA. Results: Histamine H4-receptor agonists (histamine, selected histaprodifens) increased the secretion of NGF from cultured astrocytes in a concentration-dependent manner. H4-receptor antagonists/inverse agonists (mepyramine, triprolidine) and PKC inhibitor completely blocked the effect of histamine. Histamine H2 and H4 receptor agonists did not enhance NGF secretion significantly. In addition, H2 and H3/H4 receptor antagonists did not diminish histamine-stimulated NGF release. Conclusions: Our results indicate that histamine H4 receptor and PKC are involved in the signal transduction pathway, responsible for histamine-stimulated NGF secretion from cultured astrocytes.
Deskriptorji     ASTROCYTES
NERVE GROWTH FACTORS
RECEPTORS, HISTAMINE H1
RECEPTORS, HISTAMINE H2
RECEPTORS, HISTAMINE H3
RATS, WISTAR
CELLS, CULTURED
RADIOLIGAND ASSAY
PROTEIN KINASE C
HISTAMINE
ENZYME-LINKED IMMUNOSORBENT ASSAY