Avtor/Urednik		Čučnik, Saša
Naslov		Pomen avidnosti protiteles proti beta2-glikoproteinu I pri bolnikih s sistemskimi avtoimunskimi boleznimi
Prevedeni naslov		The importance of anti-beta2-glycoprotein I antibodies' avidity in patients with systemic autoimmune diseases
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2004
Obseg		str. 81
Jezik		slo
Abstrakt		Antibodies against beta2-glycoprotein I (anti-beta2-GPI) are among the most commonly detected subsets of antiphospholipid antibodies. Nevertheless, numerous questions about methodologies of anti-beta2-GPI detection considering rational laboratory diagnostics, pathogenic mechanisms and clinical associations are still matters of intensive studies. Answers to these questions will refine their diagnostic meaning. We hypothesize that avidity of anti-beta2-GPI importantly influences their pathogenic potential and consequently also clinical relevance of laboratory findings. Affinity and cationic exchange chromatography were used to isolate beta2-GPI, polyacrylamide gel electrophoresis, N-terminal sequencing and immunochemical methods for the characterization of isolated proteins, and chaotropic enzyme-linked immunosorbent assay for the determination of avidity of IgG anti-beta2-GPI. (Abstract truncated at 2000 characters)
Deskriptorji		ANTIBODIES, ANTIPHOSPHOLIPID GLYCOPROTEINS ANTIPHOSPHOLIPID SYNDROME LUPUS ERYTHEMATOSUS, SYSTEMIC IGG CHROMATOGRAPHY, AFFINITY CHROMATOGRAPHY, ION EXCHANGE ELECTROPHORESIS, POLYACRYLAMIDE GEL AMINO ACID SEQUENCE THROMBOPHLEBITIS