Avtor/Urednik		Šimenc, Janez
Naslov		Vrednotenje elektroforeze s spreminjajočo temperaturo za identifikacijo in tipizacijo borelij lajmske borelioze
Tip		monografija
Kraj izdaje		Ljubljana
Založnik		Medicinska fakulteta
Leto izdaje		2004
Obseg		str. 75
Jezik		slo
Abstrakt		Several genospecies of the genus Borrelia are known to cause Lyme borreliosis in humans. The best studied complex is B. burgdorferi sensu lato that consist of B. af elii, B. garinii and B. burgdorferi sensu stricto. B. burgdorferii sensu lato can be isolated from various animals, humans and ticks. Humans and animals are infected by bite of an infected tick. Lyme borreliosis is a zoonosis. Lyme borreliosis is presented in various forms; skin borreliosis, neuroborreliosis, arthritis, etc. A strong correlation between geographic distribution of species B. burgdorferi sensu lato and various clinical symptoms exists. The hypothesis of organotropism is proposed: B. af elii is related to skin borreliosis, B. garinii to neuroborreliosis and B. hurgdorferi sensu stricto to Lyme arthritis. Some authors are also speculating about pathogenic variability within the species. The aim of this study was to develop an effective and simple method for typing and species identification of Borrelia burgdorferi sensu lato. We have adjusted and optimized temporal temperature gradient gel electrophoresis (TTGE) and compared the results of species identification and typing with two most widely used methods: pulsed-field gel electrophoresis (PFGE) and PCR product restriction. In total 42 isolates of Borrelia were analyzed, 40 of them from human material (skin and CSF) and two from ticks (strains B31 and ZS7). We found no difference in species identification between PFGE, TTGE and restriction of PCR product. Therefore TTGE can be used for species identification. For estimating variability of Borrelia strains within species only PFGE and TTGE are suitable. There is difference in number of different types, found by PFGE and TTGE within B. afzelii and B. burgdorferi sensu stricto, but not within B. garinii. We believe that TTGE can complement PFGE in estimating variability within Borrelia species. (Abstract truncated at 2000 characters)
Deskriptorji		LYME DISEASE BORRELIA ELECTROPHORESIS ELECTROPHORESIS, GEL, PULSED-FIELD POLYMERASE CHAIN REACTION POLYMORPHISM, RESTRICTION FRAGMENT LENGTH