Avtor/Urednik | Žolnir-Dovč, Marija | |
Naslov | Molekularno epidemiološka raziskava izolatov bakterije Mycobacterium tuberculosis v Sloveniji | |
Tip | monografija | |
Kraj izdaje | Ljubljana | |
Založnik | Univerza v Ljubljani, Medicinska fakulteta | |
Leto izdaje | 2004 | |
Obseg | str. 95 | |
Jezik | slo | |
Abstrakt | The most important factors responsible for the successful control of tuberculosis (TB) are rapid detection of TB patients, administration of adequate and efficient therapy and implementation of good contact-tracing procedures to stop further transmission. However, in the recent years DNA fingerprinting of Mycobacterium tuberculosis has given new insight into the nature of TB transmission. In a prospective nation-wide, oneyear DNA fingerprinting study, the genetic diversity of 99.7% of all M tuberculosis isolates obtained from Slovenian patients with culture-verified TB in 2001 were assessed using a standardized IS6110 restriction fragment length polymorphism (RFLP) method. To the best of our knowledge, our study represents the first nation-wide study which was performed in a country with a moderate TB incidence. Among 306 M tuberculosis isolates analysed, 228 different IS6110 RFLP patterns were found. The number of IS6110 copies varied from 2 to 16 (9.2 copies per isolate on average). Only 2 isolates (0.7%) with less than five IS6110 copies were identified. Clustered M tuberculosis isolates were detected in 116 (37.9%) patients. The degree of recent transmission in one year period was 25%. (Abstract truncated at 2000 characters) | |
Deskriptorji | MYCOBACTERIUM TUBERCULOSIS TUBERCULOSIS GENOTYPE EPIDEMIOLOGY, MOLECULAR RISK FACTORS POLYMORPHISM, RESTRICTION FRAGMENT LENGTH POLYMERASE CHAIN REACTION DIAGNOSTIC ERRORS PROSPECTIVE STUDIES SLOVENIA |