Avtor/Urednik		Trampuž, Andrej
Naslov		Izboljšanje diagnostike okužb, povezanih s tvorbo biofilmov
Prevedeni naslov		Improvement of the diagnosis of infections, associated with the formation of biofilms
Tip		članek
Vir		In: Poljak M, editor. Zbornik predavanj Okužbe vsadkov; 2004 okt 8-9; Valdoltra. Ljubljana: Slovensko zdravniško društvo,
Leto izdaje		2004
Obseg		str. 21-6
Jezik		slo
Abstrakt		Orthopedic replacement surgery has revolutionized medicine. Prosthetic joints provide millions of people with mobility and freedom from pain. Infections associated with prosthetic joints are rare, but represent the most devastating complication with high marbidity and substantial costs. Therefore, it is important to diagnose accurately prosthetic joint infection (PJI]. Current laboratory methods depend on isolation of a pathogen by culture in synovial fluid and periprosthetic tissue. However, as PJI is commonly caused by low numbers of microorganisms which are growing in biofilm, these conventional microbiology methods typically have neither ideal sensitivity nor specificity. In the recent years, two approaches to improve the diagnosis of PJI have been extensively investigated. First, various methods for removal of adherent biofilm microorganisms from surfaces of implants were used and compared. Sonication proved as one of the most efficient biofilm removal strategies. Second, molecular methods based on the detection of 16S ribosomal DNA (rDNA) by polymerase chain reaction (PCR) can additionally improve the sensitivity of PJI detection. Despite these advantages in diagnosis, an accurate diagnosis of PJI is not always easy.
Deskriptorji		PROSTHESIS-RELATED INFECTIONS BIOFILMS SYNOVIAL FLUID JOINT PROSTHESIS PROSTHESIS FAILURE POLYMERASE CHAIN REACTION