Avtor/Urednik		Šribar, Jernej; Čopič, Alenka; Poljšak-Prijatelj, Mateja; Kuret, Jošt; Logonder, Uroš; Gubenšek, Franc; Križaj, Igor
Naslov		R25 is an intracellular membrane receptor for a snake venom secretory phospholipase A21
Tip		članek
Vir		FEBS Lett
Vol. in št.		Letnik 553
Leto izdaje		2003
Obseg		str. 309-14
Jezik		eng
Abstrakt		Ammodytoxin is a presynapticaily neurotoxic (beta-neurotoxic) snake venom secretory phospholipase A2 (sPLA2). We detected a 25 kDa protein which binds the toxin with very high affinity (R25) in porcine cerebral cortex [Vučemilo et al., Biochem, Biophys. Res. Commun. 25t (1998) 202-212. Here we show that R25 is an integral membrane protein with intracellular localisation. It is the first sPLAz receptor known to date that localises to intracellular membranes. Centrifugation on sucrose gadients was used to fractionate porcine cerebral cortex. The subcellular composition of tbe fractions was determined by following the distribution of organelle-specific markers. The distribution of R25 in the fractions matched the distribution of the mitochondial marker sucinate dehydrogenase, but not the markers for plasma membrane, lysosomes, endoplasmic reticulum, synaptic and secretory vesicles. R25 most lakely resides in mitochondria, which are known to be targets for sPLAs neurotoxins in the nerve ending and are potentially implicated in the process of beta-neurotoxicity.
Deskriptorji		VIPER VENOMS SNAKE VENOMS RECEPTORS, CELL SURFACE PHOSPHOLIPASES A INTRACELLULAR MEMBRANES SWINE SUBCELLULAR FRACTIONS SUCCINATE DEHYDROGENASE PROTEIN BINDING ORGANELLES CATHEPSINS CEREBRAL CORTEX MOLECULAR WEIGHT NADPH-FERRIHEMOPROTEIN REDUCTASE 4-NITROPHENYLPHOSPHATASE