Avtor/Urednik		Ivanković, Siniša; Jurin, Mislav
Naslov		Antitumorous effects of a lentogenic Newcastle disease virus strain LaSota on mouse B16F10 melanoma
Prevedeni naslov		Protitumorsko delovanje lentogenega seva LaSota virusa bolezni Newcastle na mišji melanom B16F10
Tip		članek
Vir		Med Razgl
Vol. in št.		Letnik 43, št. Suppl 5
Leto izdaje		2004
Obseg		str. 141-8
Jezik		eng
Abstrakt		Many viruses including Newcastle disease virus (NDV), an avian pathogen, have been shown to have an inhibitory effect on some tumors. Lentogenic strain NDV LaSota is apathogenic for its natural host (poultry) and it is used as a conventional vaccine strain against Newcastle disease in birds. The aim of this study was to investigate the influence of NDV strain LaSota on the B16F10 tumor and L929 normal cells in culture as well as its effects on experimental melanoma growth in mice. In addition to the possible immunomodulation, the effect of LaSota virus on tumor-bearing mice was also investigated. In vitro results showed that LaSota virus had cytotoxic effect on mouse melanoma B16F10 cells and that the effect was dose dependent. In contrast to tumor cells, cytotoxic effects in normal L929 fibroblasts exposed to the virus were negligible. Results obtained in vivo showed that both intraperitoneal and intratumoral application of three doses of NDV LaSota can significantly inhibit B16F10 melanoma growth in mice, although a systemic intraperitoneal application was more effective than an intratumoral application. Proliferative response of splenocytes and lymph node lymphocytes from virus-treated mice to concanavalin A (ConA) exhibited some inhibitory effect 24 hours after virus application, while on day 6 after virus application, a stimulatory effect on ConA-induced lymph node lymphocyte proliferation was observed.
Izvleček		Številni virusi lahko na tumorje delujejo zaviralno. To velja tudi za virus bolezni Newcastle (NDV). Lentogeni sev LaSota NDV za svojega naravnega gostitelja (perutnina) ni patogen in se uspešno uporablja za pripravo cepiva proti bolezni Newcastle pri ptičih. Namen naše raziskave je bil, da proučimo učinke, ki jih ima sev LaSota na tumorske celice B16F10 in normalne celice L929 v kulturi in na rast eksperimentalnega melanoma pri miših. Posebej nas je zanimalo, ali ima okužba z LaSoto NDV v miših s tumorjem imunomodulatorni učinek. Virus LaSota je bil citotoksičen za mišje celice B16F10. Učinek je bil odvisen od odmerka. Normalni fibroblasti L929 so se v navzočnosti virusa normalno množili, torej virus zanje ni bil citotoksičen. Intraperitonealno ali intratumorsko dajanje virusa je pri miših občutno zavrlo rast melanoma B16F10. Intraperitonealno dajanje je bilo učinkovitejše od intratumorske injekcije. Splenociti in limfociti iz bezgavk miši, okuženih z LaSoto NDV, so se odzivali na spodbudo s konkanavalinom A manj burno kot splenociti neokuženih živali. 7aviralni učinek je bil najmočnejši 24 ur po vbrizganju virusa. Šest dni po okužbi se je zaviralni učinek izgubil in obe skupini celic sta se, kot tiste od neokuženih živali, na spodbudo s konkanavalinom A odzivali enako burno .
Deskriptorji		MELANOMA, EXPERIMENTAL ANTINEOPLASTIC AGENTS NEWCASTLE DISEASE VIRUS MICE CYTOTOXICITY, IMMUNOLOGIC DISEASE MODELS, ANIMAL